What has changed with the new K-YBGL kit format?

As the β-glucan testing landscape continues to evolve, so too has our assay methodology. In response to shifting industry needs and valuable customer feedback, we’re pleased to introduce a major update to the kit effective October 1, 2025, bringing enhanced accuracy, broader compatibility, and improved lab efficiency.

 These are some of the advantages that the newer kit provide:

• Refined Accuracy: Updated methodology improves accuracy, often yielding higher β-glucan results in mushroom samples, while maintaining assay integrity and reliability. This includes the more optimum acid hydrolysis at 30C instead of cold hydrolysis and also the introduction of a glucose correction factor that accounts for any glucose that may be lost during the acid hydrolysis. The user can prepare their own glucose correction factor, which is outlined in the protocol, or they can use the factor we have determined after numerous tests.
• Simplified Workflow: Fewer pipetting steps reduce hands-on time and lower the risk of manual error, enhancing overall ease of use.
• Broader Compatibility: Protocols now available for both liquid and solid samples.
• Reduced Reaction Time: Condensed run time: from ~ 7 h to ~ 4 h 25 min, improving lab efficiency and throughput.
• EnzyAlpha™: Optimised α-glucan degradation cocktail, now enhanced with α-amylase designed to improve performance in complex matrices. One addition of this enzyme mixture, eliminates various bottles and several pipetting steps. The kit is now a 5 vial kit instead of a 7 vial kit.
• Algal β-Glucan Measurement: Expanded validation now includes algae-derived β-glucan as a verified matrix.
• Removal of additional enzyme in the Total Glucan hydrolysis step. Due to improved acid-hydrolysis conditions, (mainly 30˚C hydrolysis instead of cold hydrolysis) the enzyme step was eliminated from the procedure. 
• An addition of a new application for samples containing maltitol. Maltitol is a newer ingredient we are seeing in b-glucan gummies so we have added an application note of how to process samples that contain maltitol. (See page 8 of the assay protocol: β-Glucan (Yeast & Mushroom) (K-YBGL) Assay Protocol)

Product page

Assay protocol