What is different between β-Glucan (Yeast & Mushroom) Assay Kit (K-YBGL) and Yeast Beta-Glucan Assay Kit (K-EBHLG)?

β-Glucan (Yeast & Mushroom) Assay Kit (K-YBL) is suitable for the measurement and analysis of 1,3:1,6-beta-glucan, 1,3-β-glucan and α-glucan in yeast, mushroom (fungi), algae and higher plants preparations.

Product Page (K-YBGL)

Please note this method is not applicable to the analysis of 1,3:1,6 β-glucan in the presence of cellulose (1,4-β-D-glucan).

K-EBHLG is no longer available. This was an enzymatic procedure for the measurement and analysis of 1,3:1,6-beta-glucan in yeast products, which have typically been extracted and prepared under acid/alkaline conditions. This kit measured 1,3-β-glucan as well. The kit did not measure beta-glucan in intact yeast cell products or in mushroom products. This is believed to be due to crosslinking of the glucan to chitin and protein. 

As a result of the wider capabilities of K-YBGL, we have refined our portfolio to concentrate on utilizing the K-YBGL for measuring 1,3:1,6 B-glucan.

For more information about this topic please see Table 1 and Figure 7 in the attached paper:

Measurement of β -Glucan in Mushrooms and Mycelial Products, McCleary & Draga, Journal of AOAC INTERNATIONAL, Volume 99, Issue 2, 1 March 2016, Pages 364–373.