The Glycerol Assay Kit reaction is a negative reaction and therefore it starts with a high absorbance of ~ 1.5 and as the reaction proceeds the absorbance decreases therefore A2 will be expected to be lower than A1 for samples containing glycerol. The more glycerol present in the sample the more the absorbance will decrease. The change in absorbance (even though this is a negative change) between readings A1 and A2 corresponds to the amount of glycerol present in the sample.
Was this article helpful?
That’s Great!
Thank you for your feedback
Sorry! We couldn't be helpful
Thank you for your feedback
Feedback sent
We appreciate your effort and will try to fix the article