In Amylazyme tablet (T-AMZ) assay procedure can centrifuging be used in the final clarification step rather than filtering? The results differ by 0.5 absorbance units.

Modified on Thu, 23 Sep, 2021 at 10:02 AM

It is necessary to filter because of the haze associated with the flour slurry. If you centrifuge all of the haze will not be removed and this gives the higher absorbance values.