When using the Xylazyme tablet method (T-XAX), within how many minutes after filtration of the reacted solution should the absorbance be measured?

When using the Xylazyme tablet method (T-XAX), filtration should be performed about 5 minutes after stopping the reaction. For Xylanases with alkaline pH optima, please use 2% tri sodium phosphate (pH 11) to stop the reaction (instead of tris base). After filtration, the solutions are stable for several hours, so there is no urgency in reading the absorbances. However, as stated above, the solutions should be filtered no more than 10 minutes after addition of the stopping reagent. The substrate is unstable in alkaline conditions, and colour will leak from the substrate leading to high blank values.

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