The choice of buffer depends on the pH optima of the endo-xylanase enzyme being measured and therefore the buffer that is closest in pH to the enzyme pH optima should be used.
For endo-xylanase with a pH optimum of approx. pH 4.5 choose buffer B, 0.1M Sodium acetate pH 4.5 plus 0.5 mg/mL BSA.
For endo-xylanase with a pH optimum of approx. pH 6.0 choose buffer D, 0.1M Sodium phosphate pH 6.0 plus 0.5 mg/mL BSA.
The supplied endo-xylanase enzyme in bottle 2, is from trichoderma source and has a pH optimum of pH 6. Hence extraction buffer/dilution buffer D is required for this enzyme.
Was this article helpful?
That’s Great!
Thank you for your feedback
Sorry! We couldn't be helpful
Thank you for your feedback
Feedback sent
We appreciate your effort and will try to fix the article