We have three soluble substrates for the measurement of xylanase activity. However we recommend using the Azo-Xylan (Birchwood) (S-AXBP or S-AXBL). All of the Azo substrates are blue coloured and the reaction is determined by adding an alcohol solution followed by centrifugation. In a petri dish assay, as the substrate is hydrolysed, it would diffuse more rapidly than the native substrate, giving zones of clearing.
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