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Why is Azo-Carob galactomannan (S-ACGLM) is prepared at 2% w/v for the assay, but carob galactomannan is used at only 0.2% w/v for the generation of the standard curve? Which concentration should I use?
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Is 590 nm the best wavelength to read at if I am using Azo-Carob galactomannan (S-ACGLM)?
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In your data sheet the Azo-Carob Galactomannan (S-ACGLM) is reported to be partially depolymerised, is this achieved by enzymatic or acid hydrolysis?
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Are Azo-Barley Glucan (S-ABG100) & Azo-Xylan the best substrates to analyse Trichoderma reseei xylanase and beta-glucanase in animal feed?
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Do you have a typical figure for the ratio of (1-3) to (1-4) linkages in the beta-glucan you use in the Azo-Barley beta-Glucan?
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What is the concentration of Azo-Barley glucan as supplied in liquid form? Also, can this substrate be used to make an agar plate pH 7.0–7.2?
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What kind of dye is used to produce Red Pullulan (S-RPUL); is it the same dye that is used in the arabinan substrate?
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Can Red Pullulan (S-RPUL) substrate be used to assay isoamylase?
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Why is 0.5 M KCI added to the substrate, Red Pullulan (S-RPUL)?
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What chromogenic substrate do you recommend to use in petri dish plates for detection of xylanase activity, and what is the principle of the method?
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Results obtained with Azo-Xylan (Birchwood) (S-AXBL / S-AXBP) seem unstable, and have dropped from 10,000 units to 850 units after 3 weeks?
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I require 4-0-Methyl-D-Glucurono-D-Xylan-Remazol Brilliant Blue R with a dye content of ~13%. Is Azo-Xylan (Birchwood) (S-AXBL / S-AXBP) suitable?