We are using your Azo-Barley Glucan (S-ABG100) solution as substrate for bacterial beta-glucanase screening. The original method uses Zn-acetate and methoxyethanol as precipitant. All other Azo-substrates use ethanol as precipitant. Why?

Modified on Thu, 23 Sep, 2021 at 4:06 PM

We use the Zn acetate in the methoxyethanol to help precipitate all the protein in malt extracts (i.e. to prevent haze). However, we have recently developed an alternative precipitant [Industrial Methylated Spirits (IMS)/Zinc acetate] for this assay. The details of this precipitant is given in the updated version of the Malt Beta Glucanase Kit booklet.

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