"Prosky" Fiber Video Method (AOAC 985.29 / AACC method 32-05.01) with K-TDFR

Modified on Thu, 2 Dec, 2021 at 2:58 PM

00:03 Introduction
02:16 Principle

Method Pre-Requisites
03:34 Preparation of Fritted Crucibles
03:52 Sample Preparation
04:50 Reagent Preparation

Procedure
05:19 Weighing of Samples
05:44 Incubation with heat stable α-amylase
07:15 Incubation with Protease
08:11 Incubation with Amyloglucosidase

Measurement of "Prosky" Total Dietary Fiber as HMWDF
09:18 Precipitation of High Molecular Weight Soluble Dietary Fiber
09:59 Filtration & Washing of Dietary Fiber (HMWDF)
12:54 Determination of "Total" Dietary Fiber (HMWDF)
14:13 Calculations

Total Dietary Fiber Assay Kit (K-TDFR)