Total Dietary Fiber Video Method (AOAC Method 991.43/AACC method 32-07.01) with K-TDFR

Modified on Tue, 7 Mar, 2023 at 10:38 AM

00:06 Introduction
02:31 Principle
04:27 Preparation of Fritted Crucibles
04:44 Sample Preparation
05:43 Reagent Preparation
06:12 Weighing of Samples
06:35 Incubation with heat stable α-amylase
08:24 Incubation with Protease
09:35 Incubation with Amyloglucosidase
10:18 Method A – Measurement of TDF as HMWDF
14:44 Method B – Separation of TDF components into IDF and SDFP
14:48 Measurement of IDF
18:01 Precipitation & Recovery of SDFP component
19:19 Calculations

Total Dietary Fiber Assay Kit (K-TDFR)