Is it possible to perform the NAD(P) coupled assays at a wavelength other than 340 nm?

Modified on Thu, 11 Nov, 2021 at 10:57 AM

Yes, it is possible to use 365 nm or 334 nm.


The calculations used in the kit booklets and the MegaCalc applications are set for readings at 340 nm [extinction coefficient for NADH of 6.3 (1 x mol-1 x cm-1)]. 


For absorbance readings at 365 nm (Hg lamp; ext. coeff. 3.4) multiply the calculated values by 1.8529.


For absorbance readings at 334 nm (Hg lamp; ext. coeff. 6.18) multiply the calculated values by 1.0194.  


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