Yes, it is possible to use 365 nm or 334 nm.
The calculations used in the kit booklets and the MegaCalc applications are set for readings at 340 nm [extinction coefficient for NADH of 6.3 (1 x mol-1 x cm-1)].
For absorbance readings at 365 nm (Hg lamp; ext. coeff. 3.4) multiply the calculated values by 1.8529.
For absorbance readings at 334 nm (Hg lamp; ext. coeff. 6.18) multiply the calculated values by 1.0194.
Was this article helpful?
That’s Great!
Thank you for your feedback
Sorry! We couldn't be helpful
Thank you for your feedback
Feedback sent
We appreciate your effort and will try to fix the article