How have you extracted the mannan (P-MANIV) from Ivory Nut?

Modified on Wed, 5 May, 2021 at 3:50 PM

The ivory meal is ground and defatted (Chloroform/methanol) and dried. It is then cooked at 80˚C in 1% HCL for 1 h. The recovered residue is then extracted with 10% NaOH overnight.