What is the factor value (F) of 54.5 used in the calculation of the Fructan Assay Kit (K-FRUC) - does it change depending on the fructose content?

Modified on Wed, 10 Nov, 2021 at 4:49 PM

The value of 54.5 is the actual amount of fructose standard used. This amount of fructose will give a certain colour in the PAHBAH assay.
Thus; F = factor = [54.5 (micrograms of fructose)] divided by the absorbance value obtained for 54.5 micrograms of fructose in the PAHBAH method.